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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A Gram stain of anaerobic growth from a peritoneal fluid abscess reveals pleomorphic, pale-staining Gram- negative rods. The isolate grows on Bacteroides Bile Esculin (BBE) agar, hydrolyzing esculin (producing black colonies), and is resistant to kanamycin (1000 µg), vancomycin (5 µg), and colistin (10 µg) disks. Which organism group is most likely?

A) Prevotella melaninogenica group
B) Fusobacterium nucleatum
C) Clostridium perfringens
D) Bacteroides fragilis group

2. A patient with HIV infection and a CD4+ T-cell count below 50 cells/µL develops a progressive neurological illness characterized by confusion, motor deficits, and seizures. Brain MRI reveals multiple white matter lesions without mass effect. PCR of the CSF is positive for JC virus. Thepathogenesis of this condition, progressive multifocal leukoencephalopathy (PML), involves:

A) Lytic infection of oligodendrocytes by JC virus, leading to demyelination.
B) Inflammatory demyelination triggered by JC virus infection of astrocytes.
C) Opportunistic infection by JC virus due to impaired cell-mediated immunity.
D) Direct cytopathic effect of JC virus on neurons.

3. A clinical microbiology laboratory is investigating a cluster of Clostridium difficile infections in a hospital.
Which of the following methods is MOST appropriate for determining if the isolates from different patients are related?

A) Gram staining and morphology
B) Molecular typing (e.g., PCR ribotyping)
C) Antimicrobial susceptibility testing
D) Enzyme immunoassay for C. difficile toxins

4. A clinical microbiology laboratory is validating a new automated blood culture system. To assess the system's ability to detect true positive results, they should:

A) Evaluate the system's performance with samples from patients with known bloodstream infections.
B) Compare the time to detection with the current manual blood culture method.
C) Spike known concentrations of various microorganisms into blood culture bottles.
D) Process a large number of sterile blood samples.

5. A patient develops a vesicular rash on their hands and feet, accompanied by fever and oral ulcers. The MOST likely causative agent is:

A) Herpes simplex virus
B) Coxsackievirus A
C) Varicella-zoster virus
D) Measles virus

Solutions: