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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes |
| Topic 2: Microbiology | 15-20% | - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Mycology, parasitology, and virology - Identification of pathogenic microorganisms |
| Topic 3: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Infectious disease serology - Immune system principles and components - Immunologic testing methodologies |
| Topic 4: Laboratory Operations | 5-10% | - Laboratory safety and regulatory compliance - Laboratory mathematics and instrumentation - Quality control and quality assurance - Pre-analytical, analytical, and post-analytical processes |
| Topic 5: Hematology | 20-25% | - White blood cell morphology and diseases - Red blood cell physiology and disorders - Routine and automated hematology procedures - Hemostasis and coagulation testing |
| Topic 6: Blood Banking (Immunohematology) | 15-20% | - Antibody detection and identification - Blood components and storage - Transfusion reactions and adverse events - Blood group systems and antigens - Compatibility testing |
| Topic 7: Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis - Urine formation and composition - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. As maturation continues in the granulocytic series the nucleus of the metamyelocyte becomes kidney or bean shaped.
Identify the cell in this illustration indicated by the arrow:
A) Metamyelocyte
B) Myeloblast
C) Promonocyte
D) Monocyte
2. Mode is defined as the number that occurs most frequently in a set of numbers.
The value that occurs most frequently in a set of results or values would be termed:
A) Mean
B) Median
C) Mode
D) Standard deviation
3. Phase of reactivity is primarily at immediate spin (4+) and reactions get weaker at AHG (w+). There is no specific pattern of reactivity and the auto control is negative which rules out an autoantibody. This is a strong cold antibody which is still slightly present after incubation and washing.
Activation and binding of the antibody takes place at room temperature or colder. Eliminating this phase will prevent the antibody from binding. Cold antibodies usually are more of a nuisance to blood bankers and are not clinically significant.
When performing an antibody screen, both the screen cells are 4+ at immediate spin and W+ at AHG. The antibody panel shows 4+ reactions at immediate spin and W+ reactions at AHG and there is no specific match to the reaction pattern. The auto control is negative. What would be a logical next step?
A) Repeat testing using warmed patient sample and reagents and just do AHG reading
B) Have patient redrawn
C) Run an enzyme panel
4. Provide the equivalent measurement for 1 centimeter.
Question options:
A) 10 millimeters
B) 1 inch
C) 1 kilometer
D) 5 millimeters
5. DAT ( Direct Antiglobulin Test ).
When AHG or Coombs serum is used to demonstrate that red cells are antibody coated in vivo, the procedure is termed:
A) Direct technique
B) Indirect technique
C) Hemagglutination technique
D) Hemolysis technique
Solutions:
| Question # 1 Answer: A | Question # 2 Answer: C | Question # 3 Answer: A | Question # 4 Answer: A | Question # 5 Answer: C |




